rabbit polyclonal anti glua3  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: GluK2 Q/R editing regulates kainate receptor signaling and long-term potentiation of AMPA receptors

doi: 10.1016/j.isci.2023.107708

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal Anti-GluA3 , Alomone , RRID: AB_2039883.

Techniques: Recombinant, Protein Extraction, Software

Journal: PLoS ONE

Article Title: AMPA Receptor Regulation at the mRNA and Protein Level in Rat Primary Cortical Cultures

doi: 10.1371/journal.pone.0025350

Figure Lengend Snippet: Double labeling of rat cortical neurons at DIV5 and DIV26 (20×) with an antibody against the glial marker GFAP (green) and with antibodies against the four AMPA receptor subunits: (A, B) GluR1; (C, D) GluR2; (E, F) GluR3 and (G, H) GluR4 (red). The images were acquired from rare regions with high density of glial cells to show that the GFAP-positive cells do not express AMPA receptors. Scale bar: 50 µm.

Article Snippet: The membranes were blocked for 60 min with 3% nonfat dry milk or 2% BSA in TBS-T (Tris-buffered saline with 0.1% Tween-20, Sigma-Aldrich) and then incubated overnight at 4°C in the blocking solution with the primary antibodies corresponding to either rabbit polyclonal anti-GluR1 (1∶200, Millipore), rabbit polyclonal anti-GluR2 (1∶2500, Millipore), rabbit polyclonal anti-GluR3 (1∶500, Alomone Labs Ltd) or rabbit polyclonal anti-GluR4 (1∶100, Millipore) with mouse monoclonal anti-GAPDH (1∶2500, Sigma-Aldrich) as a loading control.

Techniques: Labeling, Marker